ABSTRACT
Objective@#To analyze the VP1 region characteristics of coxsakievirus B4 (CoxB4) strain isolated from patients with hand, foot and mouth disease (HFMD) in Henan province and to compare the similarity and difference among these viruses isolated from different diseases.@*Methods@#The 134 samples were tested by reverse-transcription polymerase chain reaction (RT-PCR) and cell-culture-based isolation of CoxB4. The VP1 of 8 CoxB4 isolated was amplified using specific primers. The sequences were spliced, edited, analyzed and mapped into phylogenetic tree by bioinformatics software ATGC, lustalx1.83, BioEdit and MEGA4.0, respectively.@*Results@#The VP1 region length of Henan CoxB4 isolates were 852 bp. Compared with other CoxB4 strains released in GeneBank, the similarities of nucleotide and amino acid is 80.3%-97.0%, 96.1%-100%. The largest difference was 19.7% between Henan isolates and diabetes isolates, and 3.0% was the smallest difference with HFMD isolates. Phylogenetic tree analysis showed that Henan CoxB4 isolates belonged to Genotype V, and the isolates from HFMD were close to those of meningitis, distributed in a cluster. These isolates from diabetic patients were in another cluster.@*Conclusions@#There were Genotype V spreading and circulating of CoxB4 that infected HFMD patients in Henan.
ABSTRACT
TFL1 homologs play important roles in maintaining vegetative growth and inflorescence meristem identity. The plants without the function of this gene usually are flowering earlier. Their normal inflorescence development is inhibited, the inflorescence meristem eventually acquired floral identity, which producing a terminal flower. Up to now, the TFL1 homologs have been isolated from 28 species of plants, including Arabidopsis, Snapdrogen and Tomato. The phylogenic tree of TFL1 proteins is almost accordance with the relative of those plants. The inflorescence identity gene TFL1 interacted with floral meristem identity genes LFY and AP1, so as to retard the transformation from inflorescence identity into floral identity. These meristem identity genes such as TFL1 and LFY can be applied in breeding of earl-flowering cultivars, there also have plenty of potentials in breeding fruit-free plantanus, popular or willows.